Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR

Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR

In this report, we present a fluorescence-based approach to the assessment of cellular gene expression and transcription rates. Nuclear run-on was performed by supplying biotin-16-UTP to nuclei, and labeled transcripts were bound to streptavidin-coated magnetic beads. Total cDNA was then synthesized...

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Veröffentlicht in:BioTechniques. - 1993. - 29(2000), 5 vom: 01. Nov., Seite 1012-4, 1016-7
1. Verfasser: Patrone, G (VerfasserIn)
Weitere Verfasser: Puppo, F, Cusano, R, Scaranari, M, Ceccherini, I, Puliti, A, Ravazzolo, R
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers Drosophila Proteins Proto-Oncogene Proteins RNA, Messenger Tretinoin 5688UTC01R Cycloheximide 98600C0908 mehr... Proto-Oncogene Proteins c-ret EC 2.7.10.1 Receptor Protein-Tyrosine Kinases Ret protein, Drosophila Uridine Triphosphate UT0S826Z60