Poly(A) cDNA-specific (PACS) RT-PCR : a quantitative method for the measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA
We present a simple and efficient RT-PCR method for the detection and quantitation of any poly(A)-containing mRNA that is not affected by contaminating genomic DNA and does not rely on exhaustive DNase digestion protocols. The technique described here requires the use of an antisense primer designed...
| Veröffentlicht in: | BioTechniques. - 1991. - 29(2000), 4 vom: 06. Okt., Seite 762, 764-5, 766-8 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA, Complementary RNA, Messenger Poly A 24937-83-5 Superoxide Dismutase EC 1.15.1.1 |
| Zusammenfassung: | We present a simple and efficient RT-PCR method for the detection and quantitation of any poly(A)-containing mRNA that is not affected by contaminating genomic DNA and does not rely on exhaustive DNase digestion protocols. The technique described here requires the use of an antisense primer designed to contain 6-8 bp cDNA-specific sequence and an additional 17 Ts located on the 5' end to take advantage of the poly(A) tail. A second cDNA-specific sense primer can be used that does not need to be separated by intronic DNA sequence |
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| Beschreibung: | Date Completed 08.02.2001 Date Revised 28.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |