Poly(A) cDNA-specific (PACS) RT-PCR : a quantitative method for the measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA
We present a simple and efficient RT-PCR method for the detection and quantitation of any poly(A)-containing mRNA that is not affected by contaminating genomic DNA and does not rely on exhaustive DNase digestion protocols. The technique described here requires the use of an antisense primer designed...
| Veröffentlicht in: | BioTechniques. - 1991. - 29(2000), 4 vom: 06. Okt., Seite 762, 764-5, 766-8 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA, Complementary RNA, Messenger Poly A 24937-83-5 Superoxide Dismutase EC 1.15.1.1 |