Improved method for detecting differentially expressed genes using cDNA indexing

Improved method for detecting differentially expressed genes using cDNA indexing

In cDNA indexing, differentially expressed genes are identified by the display of specific, corresponding subsets of cDNA. Subdivision of the cDNA population is achieved by the sequence-specific ligation of adapters to the overhangs created by class IIS restriction enzymes. However, inadequate speci...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 28(2000), 5 vom: 01. Mai, Seite 958-64
1. Verfasser: Shaw-Smith, C J (VerfasserIn)
Weitere Verfasser: Coffey, A J, Huckle, E, Durham, J, Campbell, E A, Freeman, T C, Walters, J R, Bentley, D R
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary Oligonucleotides RNA, Messenger DNA Ligases EC 6.5.1.-