Improved method for detecting differentially expressed genes using cDNA indexing

Improved method for detecting differentially expressed genes using cDNA indexing

In cDNA indexing, differentially expressed genes are identified by the display of specific, corresponding subsets of cDNA. Subdivision of the cDNA population is achieved by the sequence-specific ligation of adapters to the overhangs created by class IIS restriction enzymes. However, inadequate speci...

Description complète

Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 28(2000), 5 vom: 01. Mai, Seite 958-64
Auteur principal: Shaw-Smith, C J (Auteur)
Autres auteurs: Coffey, A J, Huckle, E, Durham, J, Campbell, E A, Freeman, T C, Walters, J R, Bentley, D R
Format: Article
Langue:English
Publié: 2000
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA, Complementary Oligonucleotides RNA, Messenger DNA Ligases EC 6.5.1.-