Using the fluorogenic 5' nuclease assay for high-throughput detection of (CA)n repeats in radiation hybrid mapping
Here, the power of the 5' nuclease assay to detect PCR products containing (CA)n repeats was compared with that of the classical electrophoretic analysis. This assay, which relies on the use of a unique (CA)10 energy transfer-labeled probe and the 5' nuclease activity of Taq DNA polymerase...
| Publié dans: | BioTechniques. - 1991. - 28(2000), 4 vom: 19. Apr., Seite 754-8, 760-2, 764-5 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Fluorescent Dyes Taq Polymerase EC 2.7.7.- 5'-Nucleotidase EC 3.1.3.5 |