Using the fluorogenic 5' nuclease assay for high-throughput detection of (CA)n repeats in radiation hybrid mapping

Using the fluorogenic 5' nuclease assay for high-throughput detection of (CA)n repeats in radiation hybrid mapping

Here, the power of the 5' nuclease assay to detect PCR products containing (CA)n repeats was compared with that of the classical electrophoretic analysis. This assay, which relies on the use of a unique (CA)10 energy transfer-labeled probe and the 5' nuclease activity of Taq DNA polymerase...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 28(2000), 4 vom: 19. Apr., Seite 754-8, 760-2, 764-5
1. Verfasser: Jouquand, S (VerfasserIn)
Weitere Verfasser: André, C, Chéron, A, Hitte, C, Chuat, J C, Galibert, F
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Fluorescent Dyes Taq Polymerase EC 2.7.7.- 5'-Nucleotidase EC 3.1.3.5