Efficient DNA subcloning through selective restriction endonuclease digestion
Described here is a selective restriction endonuclease digestion method that eliminates the electrophoresis step that is usually used during the subcloning of new DNA sequences into typical E. coli-based plasmids. The method increases yield while decreasing laboratory resource and time utilization....
| Veröffentlicht in: | BioTechniques. - 1991. - 28(2000), 4 vom: 19. Apr., Seite 660-2, 664, 666 passim |
|---|---|
| 1. Verfasser: | |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA 9007-49-2 DNA Restriction Enzymes EC 3.1.21.- DNA Ligases EC 6.5.1.- |