Preparation of sensitive and specific oligonucleotide probes tailed using terminal transferase and dITP

Preparation of sensitive and specific oligonucleotide probes tailed using terminal transferase and dITP

An oligonucleotide probe tailed with deoxyadenosine-5'-triphosphate or deoxythymine-5'-triphosphate is detectable with high sensitivity, but has a major drawback--the tail co-hybridizes specifically to complementary sequences. This can be a problem when screening cDNA clones that contain p...

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Veröffentlicht in:BioTechniques. - 1988. - 28(2000), 3 vom: 14. März, Seite 486-90
1. Verfasser: Sugiyama, T (VerfasserIn)
Weitere Verfasser: Ishii, S, Saito, K, Yamamoto, J, Isogai, T, Ota, T
Format: Aufsatz
Sprache:English
Veröffentlicht: 2000
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Deoxyadenine Nucleotides Oligonucleotide Probes DNA Nucleotidylexotransferase EC 2.7.7.31 2'-deoxyadenosine triphosphate K8KCC8SH6N