Preparation of sensitive and specific oligonucleotide probes tailed using terminal transferase and dITP
An oligonucleotide probe tailed with deoxyadenosine-5'-triphosphate or deoxythymine-5'-triphosphate is detectable with high sensitivity, but has a major drawback--the tail co-hybridizes specifically to complementary sequences. This can be a problem when screening cDNA clones that contain p...
| Publié dans: | BioTechniques. - 1991. - 28(2000), 3 vom: 14. März, Seite 486-90 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2000
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Deoxyadenine Nucleotides Oligonucleotide Probes DNA Nucleotidylexotransferase EC 2.7.7.31 2'-deoxyadenosine triphosphate K8KCC8SH6N |