Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay)

Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay)

The antigen-dependent stabilization of an anti-hen egg lysozyme (HEL) antibody HyHEL-10 variable region was monitored with fluorescence resonance energy transfer (FRET) between fluorolabeled heavy chain (VH) and light chain (VL) fragments. The VH and VL fragments labeled with succinimide esters of f...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 27(1999), 4 vom: 01. Okt., Seite 738-42
1. Verfasser: Ueda, H (VerfasserIn)
Weitere Verfasser: Kubota, K, Wang, Y, Tsumoto, K, Mahoney, W, Kumagai, I, Nagamune, T
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Journal Article Fluorescent Dyes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region Rhodamines Muramidase EC 3.2.1.17 mehr... Fluorescein TPY09G7XIR