Homogeneous noncompetitive immunoassay based on the energy transfer between fluorolabeled antibody variable domains (open sandwich fluoroimmunoassay)
The antigen-dependent stabilization of an anti-hen egg lysozyme (HEL) antibody HyHEL-10 variable region was monitored with fluorescence resonance energy transfer (FRET) between fluorolabeled heavy chain (VH) and light chain (VL) fragments. The VH and VL fragments labeled with succinimide esters of f...
| Publié dans: | BioTechniques. - 1991. - 27(1999), 4 vom: 01. Okt., Seite 738-42 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1999
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Technical Report Journal Article Fluorescent Dyes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region Rhodamines Muramidase EC 3.2.1.17 plus... |