|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM101519699 |
| 003 |
DE-627 |
| 005 |
20231222122426.0 |
| 007 |
tu |
| 008 |
231222s1999 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed24n0339.xml
|
| 035 |
|
|
|a (DE-627)NLM101519699
|
| 035 |
|
|
|a (NLM)10219255
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Abramson, L S
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Association among somatic HPRT mutant frequency, peripheral blood T-lymphocyte clonality, and serologic parameters of disease activity in children with juvenile onset dermatomyositis
|
| 264 |
|
1 |
|c 1999
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 17.05.1999
|
| 500 |
|
|
|a Date Revised 14.11.2007
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a Somatic mutant frequencies (Mf) were determined using the HPRT T-cell cloning assay of peripheral blood T-lymphocytes from 14 children with juvenile onset dermatomyositis (JDM). Serologic parameters, specifically muscle enzyme determinations in JDM subjects, were correlated with residual lnMf (delta) in these patients to compare T-cell activation with clinical parameters associated with JDM. In addition TCR analysis was performed to determine T-cell proliferation and clonality on 12 HPRT mutant isolates from two individuals with JDM. Statistically significant correlations were found between residual lnMf and the following serologic parameters: aldolase (r = 0.771, P = 0.015); CPK (r = 0.602, P = 0.023); and SGOT (r = 0.656, P = 0.011) in children with JDM. In addition, identical TCR gene rearrangements were identified in 86 and 40% of the HPRT mutant isolates from the two patient samples analyzed, which is a significantly higher level of clonality than the 10-15% expected in normal individuals. These data suggest that determining HPRT Mf can be a useful antigen-independent method of selecting clonally expanding T-lymphocytes in autoimmune disease where relevant antigens are unknown. Future analysis of HPRT mutant isolates from children with active myositis may increase our understand of the activated T-cells involved in this disease
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, P.H.S.
|
| 650 |
|
7 |
|a Hypoxanthine Phosphoribosyltransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.4.2.8
|2 NLM
|
| 650 |
|
7 |
|a Aspartate Aminotransferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.6.1.1
|2 NLM
|
| 650 |
|
7 |
|a Creatine Kinase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.3.2
|2 NLM
|
| 650 |
|
7 |
|a Fructose-Bisphosphate Aldolase
|2 NLM
|
| 650 |
|
7 |
|a EC 4.1.2.13
|2 NLM
|
| 700 |
1 |
|
|a Albertini, R J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Pachman, L M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Finette, B A
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Clinical immunology (Orlando, Fla.)
|d 1999
|g 91(1999), 1 vom: 16. Apr., Seite 61-7
|w (DE-627)NLM098196855
|x 1521-7035
|7 nnns
|
| 773 |
1 |
8 |
|g volume:91
|g year:1999
|g number:1
|g day:16
|g month:04
|g pages:61-7
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_11
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 91
|j 1999
|e 1
|b 16
|c 04
|h 61-7
|