Activation of the human RANTES gene promoter in a macrophage cell line by lipopolysaccharide is dependent on stress-activated protein kinases and the IkappaB kinase cascade implications for exacerbation of allergic inflammation by environmental pollutants

Activation of the human RANTES gene promoter in a macrophage cell line by lipopolysaccharide is dependent on stress-activated protein kinases and the IkappaB kinase cascade : implications for exacerbation of allergic inflammation by environmental pollutants

Copyright 1999 Academic Press.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 90(1999), 3 vom: 01. März, Seite 287-301
1. Verfasser: Hiura, T S (VerfasserIn)
Weitere Verfasser: Kempiak, S J, Nel, A E
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, U.S. Gov't, P.H.S. Air Pollutants Chemokine CCL5 Hydroquinones Lipopolysaccharides NF-kappa B RNA, Messenger Transcription Factor AP-1 2-tert-butylhydroquinone mehr... C12674942B Protein Serine-Threonine Kinases EC 2.7.11.1 CHUK protein, human EC 2.7.11.10 I-kappa B Kinase IKBKB protein, human IKBKE protein, human Calcium-Calmodulin-Dependent Protein Kinases EC 2.7.11.17 JNK Mitogen-Activated Protein Kinases EC 2.7.11.24 Mitogen-Activated Protein Kinases
Beschreibung
Zusammenfassung:Copyright 1999 Academic Press.
Macrophages are targeted by environmental pollutants and play a role in allergic inflammation. We explored the molecular basis for induction of RANTES (regulated upon activation, normal T-cells expressed and secreted) mRNA by lipopolysaccharide (LPS) and the redox-active quinone, tert-butylhydroxyquinone (tBHQ). We demonstrate that transcriptional activation of the human RANTES promoter by LPS is dependent on specific AP-1 and NF-kappaB response elements, which are regulated by c-Jun N-terminal kinase (JNK) and NF-kappaB kinase cascades, respectively. The transcriptional activation of the TRE3/4 site is mediated through the transcriptional activation of c-Jun by JNK. A c-Jun mutant which lacks a transcriptional activation domain interfered in the activation of the RANTES promoter. Similarly, kinase-inactive NF-kappaB inducing kinase interfered in the activation of the RANTES promoter. While activation of the RANTES promoter could also be blocked by the downstream kinase-inactive IkappaB kinases, only IKKalpha appears to be LPS-inducible. tBHQ also exerted subtle effects on the human RANTES promoter and induced mRNA expression in parallel with generating NF-kappaB shift complexes
Beschreibung:Date Completed 13.04.1999
Date Revised 03.12.2021
published: Print
CommentIn: Clin Immunol. 1999 Mar;90(3):285-6. - PMID 10075857
Citation Status MEDLINE
ISSN:1521-7035