Single-step method for the determination of the amount of the common deletion in mitochondrial DNA

A fluorescent-based quantitative PCR assay to measure the percentage of the common deletion in rat mitochondria is reported. The amount of the common deletion is measured relative to the amount of total mitochondrial (mt)DNA. The use of a heterologous competitor construct allows the reaction to be m...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1993. - 26(1999), 2 vom: 01. Feb., Seite 290-2, 294, 296 passim
1. Verfasser: Ahmed, F (VerfasserIn)
Weitere Verfasser: Kougianos, E, Cummins, J M, Jequier, A M, Whelan, J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1999
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Research Support, Non-U.S. Gov't Technical Report Journal Article DNA Primers DNA, Mitochondrial Fluoresceins
Beschreibung
Zusammenfassung:A fluorescent-based quantitative PCR assay to measure the percentage of the common deletion in rat mitochondria is reported. The amount of the common deletion is measured relative to the amount of total mitochondrial (mt)DNA. The use of a heterologous competitor construct allows the reaction to be monitored to ensure that exponential accumulation of products occurs. The use of fluorescence-labeled primers provides a safe and sensitive means to quantify products without any adjustment for size. This assay will allow the quantitative determination of the common deletion with one dilution range
Beschreibung:Date Completed 21.04.1999
Date Revised 28.09.2018
published: Print
Citation Status MEDLINE
ISSN:0736-6205