Single-step method for the determination of the amount of the common deletion in mitochondrial DNA
A fluorescent-based quantitative PCR assay to measure the percentage of the common deletion in rat mitochondria is reported. The amount of the common deletion is measured relative to the amount of total mitochondrial (mt)DNA. The use of a heterologous competitor construct allows the reaction to be m...
| Veröffentlicht in: | BioTechniques. - 1993. - 26(1999), 2 vom: 01. Feb., Seite 290-2, 294, 296 passim |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report Journal Article DNA Primers DNA, Mitochondrial Fluoresceins |