PCR bias toward the wild-type k-ras and p53 sequences implications for PCR detection of mutations and cancer diagnosis

PCR bias toward the wild-type k-ras and p53 sequences : implications for PCR detection of mutations and cancer diagnosis

PCR-based cancer diagnosis requires detection of rare mutations in k-ras, p53 or other genes. The assumption has been that mutant and wild-type sequences amplify with near equal efficiency, so that they are eventually present in proportions representative of the starting material. Work on factor IX...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 25(1998), 4 vom: 15. Okt., Seite 684-91
1. Verfasser: Barnard, R (VerfasserIn)
Weitere Verfasser: Futo, V, Pecheniuk, N, Slattery, M, Walsh, T
Format: Aufsatz
Sprache:English
Veröffentlicht: 1998
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't CFTR protein, human DNA Primers Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6 Pfu DNA polymerase EC 2.7.7.- Taq Polymerase mehr... DNA-Directed DNA Polymerase EC 2.7.7.7