PCR bias toward the wild-type k-ras and p53 sequences : implications for PCR detection of mutations and cancer diagnosis
PCR-based cancer diagnosis requires detection of rare mutations in k-ras, p53 or other genes. The assumption has been that mutant and wild-type sequences amplify with near equal efficiency, so that they are eventually present in proportions representative of the starting material. Work on factor IX...
| Publié dans: | BioTechniques. - 1993. - 25(1998), 4 vom: 15. Okt., Seite 684-91 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1998
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| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Research Support, Non-U.S. Gov't CFTR protein, human DNA Primers Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6 Pfu DNA polymerase EC 2.7.7.- Taq Polymerase plus... |