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20231222105704.0 |
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tu |
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231222s1998 xx ||||| 00| ||eng c |
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Durward, E
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Colorimetric method for detecting amplified nucleic acids
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1 |
|c 1998
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 30.12.1998
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method requires modification of assay conditions to prevent thermal- and template-independent enzymatic activity from nonspecifically hydrolyzing dNTPs. Detection of Pi by the traditional Fiske-SubbaRow method provides a sensitivity similar to ethidium bromide staining of amplified products. The method offers a simple and rapid assay for amplified nucleic acids and can be useful in assays where confirmation of the amplified DNA product is not essential
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| 650 |
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4 |
|a Technical Report
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| 650 |
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7 |
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| 650 |
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7 |
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| 650 |
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7 |
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1 |
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|a Harris, W J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 25(1998), 4 vom: 15. Okt., Seite 608-12, 614
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
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| 773 |
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|g volume:25
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