Colorimetric method for detecting amplified nucleic acids
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method requires modification of ass...
| Veröffentlicht in: | BioTechniques. - 1988. - 25(1998), 4 vom: 15. Okt., Seite 608-12, 614 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1998
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Technical Report Deoxyadenine Nucleotides Immunoglobulin Fab Fragments Phosphates Deoxycytidine Monophosphate 1032-65-1 2'-deoxy-5'-adenosine monophosphate 653-63-4 Molybdenum 81AH48963U mehr... |
| Zusammenfassung: | Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method requires modification of assay conditions to prevent thermal- and template-independent enzymatic activity from nonspecifically hydrolyzing dNTPs. Detection of Pi by the traditional Fiske-SubbaRow method provides a sensitivity similar to ethidium bromide staining of amplified products. The method offers a simple and rapid assay for amplified nucleic acids and can be useful in assays where confirmation of the amplified DNA product is not essential |
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| Beschreibung: | Date Completed 30.12.1998 Date Revised 28.09.2018 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |