Quantitation of mixed-base populations of HIV-1 variants by automated DNA sequencing with BODIPY dye-labeled primers
Direct DNA sequencing of human immunodeficiency virus type 1 (HIV-1) pol and env gene regions was characterized for accuracy and precision. Restricted maximum likelihood (REML) analysis of molecular clone reconstruction experiments using a primer-walking strategy showed that the BODIPY and BODIPY en...
| Publié dans: | BioTechniques. - 1991. - 25(1998), 3 vom: 07. Sept., Seite 446-7, 450-2, 454, passim |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1998
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene Boron Compounds DNA Primers DNA, Viral HIV Envelope Protein gp120 DNA-Directed DNA Polymerase EC 2.7.7.7 |