Quantitation of mixed-base populations of HIV-1 variants by automated DNA sequencing with BODIPY dye-labeled primers

Direct DNA sequencing of human immunodeficiency virus type 1 (HIV-1) pol and env gene regions was characterized for accuracy and precision. Restricted maximum likelihood (REML) analysis of molecular clone reconstruction experiments using a primer-walking strategy showed that the BODIPY and BODIPY en...

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Veröffentlicht in:BioTechniques. - 1993. - 25(1998), 3 vom: 07. Sept., Seite 446-7, 450-2, 454, passim
1. Verfasser: Metzker, M L (VerfasserIn)
Weitere Verfasser: Ansari-Lari, M A, Liu, X M, Holder, D J, Gibbs, R A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1998
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene Boron Compounds DNA Primers DNA, Viral HIV Envelope Protein gp120 DNA-Directed DNA Polymerase EC 2.7.7.7