Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surve...
| Publié dans: | BioTechniques. - 1991. - 24(1998), 4 vom: 15. Apr., Seite 652-8 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1998
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA Primers RNA, Messenger Glyceraldehyde-3-Phosphate Dehydrogenases EC 1.2.1.- RNA-Directed DNA Polymerase EC 2.7.7.49 |