Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA

Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surve...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1991. - 24(1998), 4 vom: 15. Apr., Seite 652-8
1. Verfasser: Hall, L L (VerfasserIn)
Weitere Verfasser: Bicknell, G R, Primrose, L, Pringle, J H, Shaw, J A, Furness, P N
Format: Aufsatz
Sprache:English
Veröffentlicht: 1998
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't DNA Primers RNA, Messenger Glyceraldehyde-3-Phosphate Dehydrogenases EC 1.2.1.- RNA-Directed DNA Polymerase EC 2.7.7.49