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231222s1997 xx ||||| 00| ||eng c |
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|a DE-627
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|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Perry, S W
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and trypan blue staining
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| 264 |
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|c 1997
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
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|2 rdamedia
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|2 rdacarrier
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|a Date Completed 31.07.1997
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a A method for simultaneously detecting membrane permeability (characteristic of necrosis) and DNA fragmentation (characteristic of apoptosis) is described. By combining a common dye-exclusion method (Trypan Blue) with a commercially available terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL) labeling kit, we have succeeded in developing a novel methodology for obtaining permanently mounted slides of monolayer cell cultures double-labeled for DNA fragmentation and cell lysis. This method should facilitate in situ studies of cell death by allowing for a more accurate quantification of total toxicity in monolayer cell cultures and perhaps further enhance our understanding of the different mechanisms of cell death as well
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| 650 |
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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1 |
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|e verfasserin
|4 aut
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1 |
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|a Gelbard, H A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 22(1997), 6 vom: 15. Juni, Seite 1102-6
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
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|g volume:22
|g year:1997
|g number:6
|g day:15
|g month:06
|g pages:1102-6
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