Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and trypan blue staining
A method for simultaneously detecting membrane permeability (characteristic of necrosis) and DNA fragmentation (characteristic of apoptosis) is described. By combining a common dye-exclusion method (Trypan Blue) with a commercially available terminal deoxynucleotidyl transferase (TdT)-mediated dUTP...
Veröffentlicht in: | BioTechniques. - 1988. - 22(1997), 6 vom: 15. Juni, Seite 1102-6 |
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Weitere Verfasser: | , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1997
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Coloring Agents Deoxyuracil Nucleotides Reagent Kits, Diagnostic deoxyuridine triphosphate 1173-82-6 DNA Nucleotidylexotransferase EC 2.7.7.31 mehr... |