Simultaneous in situ detection of apoptosis and necrosis in monolayer cultures by TUNEL and trypan blue staining

A method for simultaneously detecting membrane permeability (characteristic of necrosis) and DNA fragmentation (characteristic of apoptosis) is described. By combining a common dye-exclusion method (Trypan Blue) with a commercially available terminal deoxynucleotidyl transferase (TdT)-mediated dUTP...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 22(1997), 6 vom: 15. Juni, Seite 1102-6
1. Verfasser: Perry, S W (VerfasserIn)
Weitere Verfasser: Epstein, L G, Gelbard, H A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Coloring Agents Deoxyuracil Nucleotides Reagent Kits, Diagnostic deoxyuridine triphosphate 1173-82-6 DNA Nucleotidylexotransferase EC 2.7.7.31 mehr... Trypan Blue I2ZWO3LS3M