Epitope mapping and tagging by recombination PCR mutagenesis
We describe a rapid PCR method that directly inserts an epitope tag into an open reading frame (ORF) to facilitate protein detection. This project was performed within a varicella-zoster virus (VZV) system. In earlier work, we produced a monoclonal antibody (MAb 3B3) to one VZV ORF called gE. MAb 3B...
| Publié dans: | BioTechniques. - 1991. - 22(1997), 2 vom: 31. Feb., Seite 332-7 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1997
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, U.S. Gov't, P.H.S. Antibodies, Monoclonal Epitopes Viral Envelope Proteins glycoprotein E, varicella-zoster virus |