Epitope mapping and tagging by recombination PCR mutagenesis

Epitope mapping and tagging by recombination PCR mutagenesis

We describe a rapid PCR method that directly inserts an epitope tag into an open reading frame (ORF) to facilitate protein detection. This project was performed within a varicella-zoster virus (VZV) system. In earlier work, we produced a monoclonal antibody (MAb 3B3) to one VZV ORF called gE. MAb 3B...

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Veröffentlicht in:BioTechniques. - 1991. - 22(1997), 2 vom: 31. Feb., Seite 332-7
1. Verfasser: Hatfield, C (VerfasserIn)
Weitere Verfasser: Duus, K M, Chen, J, Jones, D H, Grose, C
Format: Aufsatz
Sprache:English
Veröffentlicht: 1997
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, U.S. Gov't, P.H.S. Antibodies, Monoclonal Epitopes Viral Envelope Proteins glycoprotein E, varicella-zoster virus