Efficient long-PCR site-specific mutagenesis of a high GC template
A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...
| Publié dans: | BioTechniques. - 1993. - 21(1996), 3 vom: 15. Sept., Seite 472-4, 476-8, 480 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1996
|
| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA, Recombinant UL53 protein, Human herpesvirus 1 Viral Proteins Viral Structural Proteins Guanine 5Z93L87A1R Cytosine plus... |