Efficient long-PCR site-specific mutagenesis of a high GC template

Efficient long-PCR site-specific mutagenesis of a high GC template

A long PCR method was developed for the efficient site-specific mutagenesis of herpes simplex virus (HSV-1) DNA fragments with high GC content. In this protocol, a PCR product was partially extended first using a cloned DNA fragment. The final mutagenized fragment was produced after a second extensi...

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Veröffentlicht in:BioTechniques. - 1993. - 21(1996), 3 vom: 15. Sept., Seite 472-4, 476-8, 480
1. Verfasser: Chouljenko, V (VerfasserIn)
Weitere Verfasser: Jayachandra, S, Rybachuk, G, Kousoulas, K G
Format: Aufsatz
Sprache:English
Veröffentlicht: 1996
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. DNA, Recombinant UL53 protein, Human herpesvirus 1 Viral Proteins Viral Structural Proteins Guanine 5Z93L87A1R Cytosine mehr... 8J337D1HZY DNA polymerase, Thermus thermophilus EC 2.7.7.- Tli polymerase DNA-Directed DNA Polymerase EC 2.7.7.7 endodeoxyribonuclease SnaBI EC 3.1.21.- Deoxyribonucleases, Type II Site-Specific EC 3.1.21.4