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|a eng
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1 |
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|a Andersson, B
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Method for 96-well M13 DNA template preparations for large-scale sequencing
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|c 1996
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 26.12.1996
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|a Date Revised 30.11.2018
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|a published: Print
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|a Citation Status MEDLINE
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|a Efficient preparation of DNA templates is an important step in large-scale DNA sequencing. The ensure high-quality sequence data, we have prepared M13 phage DNA templates using a glass fiber-filtration method. We present the adaptation of this protocol to a 96-well format using commercially available filter plates. Two variations are described: one using polyethylene glycol precipitation and a second where the phage particles are disrupted before filtration, thus eliminating the need for precipitation. Using either of these protocols, 96 templates can be prepared in less than 2 h. Sufficient DNA for 1-2 dye primer sequencing reactions is routinely obtained from 1 mL of culture, and the resulting sequence data are of high quality
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| 650 |
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|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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|a Technical Report
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4 |
|a Journal Article
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7 |
|a DNA, Viral
|2 NLM
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| 650 |
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7 |
|a Polyethylene Glycols
|2 NLM
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| 650 |
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7 |
|a 3WJQ0SDW1A
|2 NLM
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| 700 |
1 |
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|a Lu, J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Edwards, K E
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Muzny, D M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gibbs, R A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 20(1996), 6 vom: 01. Juni, Seite 1022-7
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
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