Method for 96-well M13 DNA template preparations for large-scale sequencing
Efficient preparation of DNA templates is an important step in large-scale DNA sequencing. The ensure high-quality sequence data, we have prepared M13 phage DNA templates using a glass fiber-filtration method. We present the adaptation of this protocol to a 96-well format using commercially availabl...
| Veröffentlicht in: | BioTechniques. - 1993. - 20(1996), 6 vom: 01. Juni, Seite 1022-7 |
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| Weitere Verfasser: | , , , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1996
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA, Viral Polyethylene Glycols 3WJQ0SDW1A |
| Zusammenfassung: | Efficient preparation of DNA templates is an important step in large-scale DNA sequencing. The ensure high-quality sequence data, we have prepared M13 phage DNA templates using a glass fiber-filtration method. We present the adaptation of this protocol to a 96-well format using commercially available filter plates. Two variations are described: one using polyethylene glycol precipitation and a second where the phage particles are disrupted before filtration, thus eliminating the need for precipitation. Using either of these protocols, 96 templates can be prepared in less than 2 h. Sufficient DNA for 1-2 dye primer sequencing reactions is routinely obtained from 1 mL of culture, and the resulting sequence data are of high quality |
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| Beschreibung: | Date Completed 26.12.1996 Date Revised 30.11.2018 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |