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|a eng
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| 100 |
1 |
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|a Gaunitz, F
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation
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| 264 |
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1 |
|c 1996
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 26.09.1996
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| 500 |
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|a Date Revised 28.09.2018
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a We present a detailed protocol for the transient transfection of non-proliferating primary cultured hepatocytes that is easily reproducible. Using a modification of the classical CaPO4/DNA precipitation method, this protocol is an inexpensive alternative to other methods that are often cumbersome, expensive, difficult to reproduce or harmful to primary hepatocytes. Because only 0.5 x 10(6) cells are needed for a single transfection experiment, several reporter genes can be introduced into hepatocytes of a single liver preparation. With our protocol, different plasmids can be introduced into one cell. In this way, cis-trans interactions can be examined and reporter gene expression can be normalized for transfection efficiency. Furthermore, we describe details of a transfection experiment with two different reporter gene vectors using a luciferase gene and a lacZ gene. The results presented may be helpful to other groups concerned with improved timing of transfection experiments
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| 700 |
1 |
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|a Papke, M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gebhardt, R
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 20(1996), 5 vom: 01. Mai, Seite 826-30, 832
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
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|g volume:20
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