Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation
We present a detailed protocol for the transient transfection of non-proliferating primary cultured hepatocytes that is easily reproducible. Using a modification of the classical CaPO4/DNA precipitation method, this protocol is an inexpensive alternative to other methods that are often cumbersome, e...
Veröffentlicht in: | BioTechniques. - 1988. - 20(1996), 5 vom: 01. Mai, Seite 826-30, 832 |
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Weitere Verfasser: | , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1996
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Journal Article Calcium Phosphates alpha-tricalcium phosphate tetracalcium phosphate calcium phosphate, monobasic, anhydrous 701EKV9RMN DNA 9007-49-2 calcium phosphate 97Z1WI3NDX mehr... |