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tu |
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231222s1993 xx ||||| 00| ||eng c |
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|a eng
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| 100 |
1 |
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|a Turnbow, M A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Ribonuclease protection assay
|b use of biotinylated probes for the detection of two messenger RNAs
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| 264 |
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1 |
|c 1993
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 20.10.1993
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| 500 |
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|a Date Revised 21.11.2013
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a A nonradioactive modification of the traditional ribonuclease protection assay is described and applied to the detection of two messenger RNAs. The biotinylated probes are stable and easy to handle, which permits the processing of large numbers of samples. Chemiluminescence is generated with streptavidin-conjugated alkaline phosphatase and provides sensitivity equal to or greater than that achieved with the radioactive detection method
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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4 |
|a Technical Report
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4 |
|a Journal Article
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| 650 |
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7 |
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| 650 |
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7 |
|a Insulin Receptor Substrate Proteins
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| 650 |
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7 |
|a Irs1 protein, mouse
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| 650 |
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7 |
|a Phosphoproteins
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| 650 |
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7 |
|a RNA Probes
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
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| 650 |
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7 |
|a Biotin
|2 NLM
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| 650 |
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7 |
|a 6SO6U10H04
|2 NLM
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| 650 |
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7 |
|a Ribonucleases
|2 NLM
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| 650 |
|
7 |
|a EC 3.1.-
|2 NLM
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| 700 |
1 |
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|a Garner, C W
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1993
|g 15(1993), 2 vom: 01. Aug., Seite 267-70
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:15
|g year:1993
|g number:2
|g day:01
|g month:08
|g pages:267-70
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|d 15
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