Ribonuclease protection assay : use of biotinylated probes for the detection of two messenger RNAs
A nonradioactive modification of the traditional ribonuclease protection assay is described and applied to the detection of two messenger RNAs. The biotinylated probes are stable and easy to handle, which permits the processing of large numbers of samples. Chemiluminescence is generated with strepta...
| Veröffentlicht in: | BioTechniques. - 1993. - 15(1993), 2 vom: 01. Aug., Seite 267-70 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Actins Insulin Receptor Substrate Proteins Irs1 protein, mouse Phosphoproteins RNA Probes RNA, Messenger Biotin mehr... |
| Zusammenfassung: | A nonradioactive modification of the traditional ribonuclease protection assay is described and applied to the detection of two messenger RNAs. The biotinylated probes are stable and easy to handle, which permits the processing of large numbers of samples. Chemiluminescence is generated with streptavidin-conjugated alkaline phosphatase and provides sensitivity equal to or greater than that achieved with the radioactive detection method |
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| Beschreibung: | Date Completed 20.10.1993 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |