Efficacy of fluorescence-based PCR-SSCP for detection of point mutations
We assessed the efficiency of fluorescence-based PCR single-strand conformation polymorphism analysis (PCR-SSCP) using an automated DNA sequencer and analysis software. We evaluated 48 point mutations in a 191-bp stretch of mouse beta-globin. The mutations included 10 transversions and 38 transition...
| Veröffentlicht in: | BioTechniques. - 1991. - 15(1993), 4 vom: 01. Okt., Seite 684-91 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1993
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA, Single-Stranded Fluorescent Dyes Globins 9004-22-2 DNA 9007-49-2 Deoxyribonuclease EcoRI EC 3.1.21.- |