|
|
|
|
| LEADER |
01000caa a22002652c 4500 |
| 001 |
NLM082260567 |
| 003 |
DE-627 |
| 005 |
20250130042149.0 |
| 007 |
tu |
| 008 |
231222s1993 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0275.xml
|
| 035 |
|
|
|a (DE-627)NLM082260567
|
| 035 |
|
|
|a (NLM)8251171
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Ellison, J
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Efficacy of fluorescence-based PCR-SSCP for detection of point mutations
|
| 264 |
|
1 |
|c 1993
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
|
| 338 |
|
|
|a Band
|b nc
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 07.01.1994
|
| 500 |
|
|
|a Date Revised 01.05.2006
|
| 500 |
|
|
|a published: Print
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a We assessed the efficiency of fluorescence-based PCR single-strand conformation polymorphism analysis (PCR-SSCP) using an automated DNA sequencer and analysis software. We evaluated 48 point mutations in a 191-bp stretch of mouse beta-globin. The mutations included 10 transversions and 38 transitions; and both types of mutation were compared at six different locations in the PCR fragment. Mobilities of the red dye-labeled internal standard fragments were non-proportional to size but highly reproducible and were used to normalize or correct minor differences in apparent electrophoretic mobility between lanes. Forty-six of forty-eight mutants (96%) were distinguished from wild type when run in separate lanes using one set of conditions. Co-electrophoresis of wild type and mutant in the same lane resolved 100% of 45 mutants from wild type. Under conditions of this study, the magnitude of mobility shifts resulting from the globin mutations were largely determined by position of the mutation, rather than by the nature of the substitution (transition vs. transversion). However, examination of paired mutations at the same position revealed that some substitutions cause greater mobility shifts than others
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
7 |
|a DNA, Single-Stranded
|2 NLM
|
| 650 |
|
7 |
|a Fluorescent Dyes
|2 NLM
|
| 650 |
|
7 |
|a Globins
|2 NLM
|
| 650 |
|
7 |
|a 9004-22-2
|2 NLM
|
| 650 |
|
7 |
|a DNA
|2 NLM
|
| 650 |
|
7 |
|a 9007-49-2
|2 NLM
|
| 650 |
|
7 |
|a Deoxyribonuclease EcoRI
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.21.-
|2 NLM
|
| 700 |
1 |
|
|a Dean, M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Goldman, D
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 15(1993), 4 vom: 01. Okt., Seite 684-91
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
|
| 773 |
1 |
8 |
|g volume:15
|g year:1993
|g number:4
|g day:01
|g month:10
|g pages:684-91
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_21
|
| 912 |
|
|
|a GBV_ILN_22
|
| 912 |
|
|
|a GBV_ILN_24
|
| 912 |
|
|
|a GBV_ILN_39
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_50
|
| 912 |
|
|
|a GBV_ILN_60
|
| 912 |
|
|
|a GBV_ILN_62
|
| 912 |
|
|
|a GBV_ILN_65
|
| 912 |
|
|
|a GBV_ILN_70
|
| 912 |
|
|
|a GBV_ILN_99
|
| 912 |
|
|
|a GBV_ILN_121
|
| 912 |
|
|
|a GBV_ILN_130
|
| 912 |
|
|
|a GBV_ILN_227
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_618
|
| 912 |
|
|
|a GBV_ILN_640
|
| 912 |
|
|
|a GBV_ILN_754
|
| 912 |
|
|
|a GBV_ILN_2001
|
| 912 |
|
|
|a GBV_ILN_2002
|
| 912 |
|
|
|a GBV_ILN_2003
|
| 912 |
|
|
|a GBV_ILN_2005
|
| 912 |
|
|
|a GBV_ILN_2006
|
| 912 |
|
|
|a GBV_ILN_2007
|
| 912 |
|
|
|a GBV_ILN_2008
|
| 912 |
|
|
|a GBV_ILN_2009
|
| 912 |
|
|
|a GBV_ILN_2010
|
| 912 |
|
|
|a GBV_ILN_2012
|
| 912 |
|
|
|a GBV_ILN_2015
|
| 912 |
|
|
|a GBV_ILN_2018
|
| 912 |
|
|
|a GBV_ILN_2023
|
| 912 |
|
|
|a GBV_ILN_2035
|
| 912 |
|
|
|a GBV_ILN_2040
|
| 912 |
|
|
|a GBV_ILN_2060
|
| 912 |
|
|
|a GBV_ILN_2099
|
| 912 |
|
|
|a GBV_ILN_2105
|
| 912 |
|
|
|a GBV_ILN_2121
|
| 912 |
|
|
|a GBV_ILN_2470
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 15
|j 1993
|e 4
|b 01
|c 10
|h 684-91
|