Tethered bandshift assay and affinity purification of a new DNA-binding protein
DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshift...
| Veröffentlicht in: | BioTechniques. - 1991. - 16(1994), 5 vom: 18. Mai, Seite 852-5 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Technical Report Journal Article DNA-Binding Proteins |
| Zusammenfassung: | DNA-binding proteins can be purified by their affinity for probes with specific sequences that are tethered to magnetic beads. A restriction site at the end of the tether allows release of probe with factor still on it. This probe with bound factor can be bandshifted directly to compare to bandshifts in whole extracts. This method correlates purified factor seen indirectly by bandshift with factor that is purified and released, because they are the same |
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| Beschreibung: | Date Completed 27.09.1994 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |