Rapid silver staining and recovery of PCR products separated on polyacrylamide gels
A rapid silver-staining procedure for DNA fragments in polyacrylamide gels is described. The time required for band detection is 15 min and the limit of sensitivity 3 pg/mm2. PCR products subjected to this rapid staining protocol are readily recovered from the gel by excision and elution by incubati...
| Veröffentlicht in: | BioTechniques. - 1988. - 17(1994), 5 vom: 01. Nov., Seite 914-21 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Research Support, Non-U.S. Gov't Technical Report Journal Article DNA, Helminth |
| Zusammenfassung: | A rapid silver-staining procedure for DNA fragments in polyacrylamide gels is described. The time required for band detection is 15 min and the limit of sensitivity 3 pg/mm2. PCR products subjected to this rapid staining protocol are readily recovered from the gel by excision and elution by incubation at 95 degrees C for 20 min. Bands of up to 3 kb have been recovered and reamplified from either recently prepared or dried gels. The rapid staining protocol significantly decreases the processing time required for silver-stained polyacrylamide gels, which is of particular importance in diagnostic situations. The recovery protocol allows individual bands from complex mixtures to be easily recovered for sequencing or probe preparation |
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| Beschreibung: | Date Completed 09.03.1995 Date Revised 08.04.2022 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |