Rapid silver staining and recovery of PCR products separated on polyacrylamide gels

A rapid silver-staining procedure for DNA fragments in polyacrylamide gels is described. The time required for band detection is 15 min and the limit of sensitivity 3 pg/mm2. PCR products subjected to this rapid staining protocol are readily recovered from the gel by excision and elution by incubati...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 17(1994), 5 vom: 01. Nov., Seite 914-21
1. Verfasser: Sanguinetti, C J (VerfasserIn)
Weitere Verfasser: Dias Neto, E, Simpson, A J
Format: Aufsatz
Sprache:English
Veröffentlicht: 1994
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Comparative Study Research Support, Non-U.S. Gov't Technical Report Journal Article DNA, Helminth