A versatile and general prokaryotic expression vector, pLACT7
We have previously reported the constitutive over-expression of the tRNA-guanine transglycosylase (TGT) from plasmid pTGT1 in Escherichia coli. To obtain a controllable expression system for TGT, we have subsequently cloned the tgt gene into pET21b. Though the overexpression of TGT is inducible in p...
| Publié dans: | BioTechniques. - 1991. - 17(1994), 4 vom: 03. Okt., Seite 686, 688, 690-1 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1994
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Pentosyltransferases EC 2.4.2.- queuine tRNA-ribosyltransferase EC 2.4.2.29 |
| Résumé: | We have previously reported the constitutive over-expression of the tRNA-guanine transglycosylase (TGT) from plasmid pTGT1 in Escherichia coli. To obtain a controllable expression system for TGT, we have subsequently cloned the tgt gene into pET21b. Though the overexpression of TGT is inducible in pET21b, the plasmid has a low copy number, a poor yield of single-stranded DNA and relies on an E. coli strain that produces T7 RNA polymerase for protein expression. We have combined the features of pTZ18U and pET21b and have constructed a versatile plasmid pLACT7 that has a high copy number, a high yield of single-stranded DNA and both the T7 and lac promoters for protein expression in a wide variety of E. coli strains |
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| Description: | Date Completed 01.03.1995 Date Revised 14.11.2007 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |