A versatile and general prokaryotic expression vector, pLACT7
We have previously reported the constitutive over-expression of the tRNA-guanine transglycosylase (TGT) from plasmid pTGT1 in Escherichia coli. To obtain a controllable expression system for TGT, we have subsequently cloned the tgt gene into pET21b. Though the overexpression of TGT is inducible in p...
| Veröffentlicht in: | BioTechniques. - 1991. - 17(1994), 4 vom: 03. Okt., Seite 686, 688, 690-1 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1994
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article Pentosyltransferases EC 2.4.2.- queuine tRNA-ribosyltransferase EC 2.4.2.29 |