A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with un...
| Publié dans: | BioTechniques. - 1991. - 18(1995), 3 vom: 01. März, Seite 490-7 |
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| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article |
| Langue: | English |
| Publié: |
1995
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Carbocyanines Connexin 43 Fluoresceins Fluorescent Dyes Succinates 3,3'-dioctadecylindocarbocyanine 40957-95-7 Oncogene Protein pp60(v-src) plus... |
| Résumé: | We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time |
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| Description: | Date Completed 20.07.1995 Date Revised 21.11.2013 published: Print ErratumIn: Biotechniques 1995 Aug;19(2):212 Citation Status MEDLINE |
| ISSN: | 1940-9818 |