A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with un...
| Veröffentlicht in: | BioTechniques. - 1991. - 18(1995), 3 vom: 01. März, Seite 490-7 |
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| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1995
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Carbocyanines Connexin 43 Fluoresceins Fluorescent Dyes Succinates 3,3'-dioctadecylindocarbocyanine 40957-95-7 Oncogene Protein pp60(v-src) mehr... |