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01000caa a22002652c 4500 |
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DE-627 |
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20250129115658.0 |
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tu |
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231222s1993 xx ||||| 00| ||eng c |
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|a pubmed25n0256.xml
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|a (DE-627)NLM07668881X
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|a (NLM)7692896
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Chomczynski, P
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples
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| 264 |
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1 |
|c 1993
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 26.11.1993
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|a Date Revised 09.01.2024
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a This report describes a new method for simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. The method is based on the use of a reagent containing phenol and guanidine thiocyanate. A biological sample is homogenized in the reagent and the simultaneous isolation of RNA, DNA and proteins is accomplished in a single step by a liquid-phase separation. The isolation of RNA can be completed in about 1 h, and DNA and proteins in about 3 h. The simultaneously isolated RNA, DNA and proteins are ready for Northern, Southern and Western blotting. The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Guanidines
|2 NLM
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| 650 |
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7 |
|a Indicators and Reagents
|2 NLM
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| 650 |
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7 |
|a Phenols
|2 NLM
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| 650 |
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7 |
|a Proteins
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a Thiocyanates
|2 NLM
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| 650 |
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7 |
|a Phenol
|2 NLM
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| 650 |
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7 |
|a 339NCG44TV
|2 NLM
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| 650 |
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7 |
|a guanidine thiocyanate
|2 NLM
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| 650 |
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7 |
|a 593-84-0
|2 NLM
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| 650 |
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7 |
|a RNA
|2 NLM
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| 650 |
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7 |
|a 63231-63-0
|2 NLM
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 650 |
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7 |
|a Thyroglobulin
|2 NLM
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| 650 |
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7 |
|a 9010-34-8
|2 NLM
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| 650 |
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7 |
|a Lactalbumin
|2 NLM
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| 650 |
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7 |
|a 9013-90-5
|2 NLM
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 15(1993), 3 vom: 05. Sept., Seite 532-4, 536-7
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
|
| 773 |
1 |
8 |
|g volume:15
|g year:1993
|g number:3
|g day:05
|g month:09
|g pages:532-4, 536-7
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