A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples
This report describes a new method for simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. The method is based on the use of a reagent containing phenol and guanidine thiocyanate. A biological sample is homogenized in the reagent and the simultaneous isolation of RNA, DNA a...
| Publié dans: | BioTechniques. - 1991. - 15(1993), 3 vom: 05. Sept., Seite 532-4, 536-7 |
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| Auteur principal: | |
| Format: | Article |
| Langue: | English |
| Publié: |
1993
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Guanidines Indicators and Reagents Phenols Proteins RNA, Messenger Thiocyanates Phenol 339NCG44TV guanidine thiocyanate plus... |
| Résumé: | This report describes a new method for simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. The method is based on the use of a reagent containing phenol and guanidine thiocyanate. A biological sample is homogenized in the reagent and the simultaneous isolation of RNA, DNA and proteins is accomplished in a single step by a liquid-phase separation. The isolation of RNA can be completed in about 1 h, and DNA and proteins in about 3 h. The simultaneously isolated RNA, DNA and proteins are ready for Northern, Southern and Western blotting. The complete recovery of DNA from samples used for the RNA and protein isolation makes it possible to normalize the results of gene expression studies based on DNA content instead of on the more variable total RNA, protein content or tissue weight |
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| Description: | Date Completed 26.11.1993 Date Revised 09.01.2024 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |