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tu |
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231222s1995 xx ||||| 00| ||eng c |
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| 041 |
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|a eng
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| 100 |
1 |
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|a Sun, H W
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Salvaging recombinants from low-efficiency ligase reactions for more efficient subcloning
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| 264 |
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1 |
|c 1995
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
|
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 09.08.1995
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| 500 |
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|a Date Revised 14.11.2007
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a Certain types of ligase reactions can be problematic, such as those involving PCR products, blunt-ends and multiple DNA inserts. A simple PCR-based strategy was developed to overcome cloning difficulties with these inefficient ligase reactions. After an initial ligase reaction, primers complementary to the vector are utilized to amplify the DNA fragment from (the few) successful recombinants in the ligation mixture. This DNA fragment is processed for use in a more conventional and straightforward ligase reaction. We demonstrate the potential of the technique by applying it to a variety of difficult ligase reactions
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, P.H.S.
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| 650 |
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4 |
|a Technical Report
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| 650 |
|
7 |
|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a DNA, Recombinant
|2 NLM
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| 650 |
|
7 |
|a Receptors, Erythropoietin
|2 NLM
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| 650 |
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7 |
|a Ligases
|2 NLM
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| 650 |
|
7 |
|a EC 6.-
|2 NLM
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| 700 |
1 |
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|a Lolis, E
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 18(1995), 4 vom: 15. Apr., Seite 644-6, 648, 650
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnns
|
| 773 |
1 |
8 |
|g volume:18
|g year:1995
|g number:4
|g day:15
|g month:04
|g pages:644-6, 648, 650
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|d 18
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|h 644-6, 648, 650
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