Salvaging recombinants from low-efficiency ligase reactions for more efficient subcloning
Certain types of ligase reactions can be problematic, such as those involving PCR products, blunt-ends and multiple DNA inserts. A simple PCR-based strategy was developed to overcome cloning difficulties with these inefficient ligase reactions. After an initial ligase reaction, primers complementary...
| Publié dans: | BioTechniques. - 1991. - 18(1995), 4 vom: 15. Apr., Seite 644-6, 648, 650 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1995
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| Accès à la collection: | BioTechniques |
| Sujets: | Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Technical Report Journal Article DNA Primers DNA, Recombinant Receptors, Erythropoietin Ligases EC 6.- |