Highly sensitive northern hybridization of rare mRNA using a positively charged nylon membrane
The effect of different RNA fixation methods using Hybond-N and Hybond-N+ nylon membranes in Northern blot experiments was analyzed with human glyceraldehyde-3-phosphate dehydrogenase and human interleukin-3 receptor alpha-subunit as radioactive labeled DNA probes. Highest sensitivity could be achie...
Veröffentlicht in: | BioTechniques. - 1988. - 16(1994), 6 vom: 30. Juni, Seite 1074-8 |
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Weitere Verfasser: | , |
Format: | Aufsatz |
Sprache: | English |
Veröffentlicht: |
1994
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Zugriff auf das übergeordnete Werk: | BioTechniques |
Schlagworte: | Technical Report Journal Article DNA Probes Membranes, Artificial Nylons RNA, Messenger Receptors, Interleukin-3 RNA 63231-63-0 Glyceraldehyde-3-Phosphate Dehydrogenases |