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20250129073949.0 |
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tu |
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231222s1994 xx ||||| 00| ||eng c |
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|a (NLM)7521187
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Beckers, T
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Highly sensitive northern hybridization of rare mRNA using a positively charged nylon membrane
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1 |
|c 1994
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 06.10.1994
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|a Date Revised 17.04.2006
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|a published: Print
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|a Citation Status MEDLINE
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|a The effect of different RNA fixation methods using Hybond-N and Hybond-N+ nylon membranes in Northern blot experiments was analyzed with human glyceraldehyde-3-phosphate dehydrogenase and human interleukin-3 receptor alpha-subunit as radioactive labeled DNA probes. Highest sensitivity could be achieved by mild alkaline fixation of RNA to positively charged Hybond-N+ nylon membrane. Fixation by UV light resulted in reduced sensitivity in comparison with alkaline treatment. Fixation of RNA to Hybond-N or -N+ membranes by baking for 2 h at 80 degrees C resulted in a strong decrease in sensitivity and should be avoided when using modified nylon membranes in Northern hybridizations
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|a Technical Report
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|a Journal Article
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|a DNA Probes
|2 NLM
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7 |
|a Membranes, Artificial
|2 NLM
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| 650 |
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7 |
|a Nylons
|2 NLM
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| 650 |
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7 |
|a RNA, Messenger
|2 NLM
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| 650 |
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7 |
|a Receptors, Interleukin-3
|2 NLM
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| 650 |
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7 |
|a RNA
|2 NLM
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| 650 |
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7 |
|a 63231-63-0
|2 NLM
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| 650 |
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|a Glyceraldehyde-3-Phosphate Dehydrogenases
|2 NLM
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| 650 |
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|a EC 1.2.1.-
|2 NLM
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| 700 |
1 |
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|a Schmidt, P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hilgard, P
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 16(1994), 6 vom: 30. Juni, Seite 1074-8
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:16
|g year:1994
|g number:6
|g day:30
|g month:06
|g pages:1074-8
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|d 16
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|h 1074-8
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